In particular we present protocols to study the PC biogenesis and function in 2D neural. 3D celltissue models. Generating 3d Human Cardiac Constructs From Pluripotent Stem Cells Ebiomedicine Most research regarding cancer biology is based on experiments using two-dimensional 2D cell cultures in vitroHowever 2D cultures have many limitations such as the. . This review will focus on the use of stem cells particularly hiPSCs to model neurodegenerative diseases. Ad Only for Research. 2D and 3D Stem Cell Models of Primate Cortical Development Identify Species-Specific Differences in Progenitor Behavior Contributing to Brain Size. From iPSC MSC to 3D Bioprinting Organoid. Reasons for the increasing acceptance and use of 3D cell cultures include. 2D and 3D Stem Cell Models of Primate Cortical Development Identify Species-Specific Differences in Progenitor Behavior Contributing to Brain Size. Human pluripotent ste...